In the course of a engagement to Lichfield Cathedral on the start of the week, the King was heckled by a individual who questioned him about Prince Andrew's connections with disgraced financier Jeffrey Epstein.
The man asked how long the monarch had been known about his sibling's association with Epstein, and also questioned whether he had asked the police to "cover up" for Andrew.
Amid the protest, there were crowds of fans and cheers for the King, who was visiting Staffordshire for an ceremony.
The Duke of York's links with Jeffrey Epstein have been under close examination in recent weeks. The prince, who gave up his titles earlier this month, has repeatedly denied any wrongdoing.
"How long have you been aware about Andrew and Jeffrey Epstein? Did you request the authorities to cover up for Andrew?"
The King ignored the questions but carried on greeting supporters, some of whom shouted "God Save The King". One supporter can be heard asking him for a embrace.
A female bystander outside the cathedral was also observed telling the heckler to "go away" and to "shut up".
The controversy over the Duke of York's connections to Jeffrey Epstein has been revived by misconduct claims detailed in Virginia Giuffre's posthumously-published memoir.
In the publication, the accuser says she had sexual encounters with the prince on three separate occasions, including once with Epstein and "eight other young girls"
Andrew, who reached a out-of-court agreement with the plaintiff in 2022, has consistently denied any wrongdoing. In 2019, he told a news program he did not remember meeting Ms Giuffre "ever", and that they "never had any sort of intimate relations."
But recently, fresh questions have been asked about how he is able to fund his lifestyle despite not being a active member of the royal family.
The disclosure that he has only ever paid a token annual rent on his Windsor mansion has intensified the scrutiny over his housing situation - even though he made significant contributions up front, including for improvements.
The political party said he should be called in front of MPs to give testimony about his rental agreement of his lodge.
But last week, the government declined to give parliamentarians time to discuss the royal's titles or his residence, despite growing pressure for scrutiny.
Buckingham Palace has not issued a statement on whether Prince Andrew might relocate of his home - or where he might go.
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